Figure 1. Reverse transcription–polymerase chain reaction (RT–PCR) analysis of VEGF-A and VEGF-C. RT–PCR analysis of VEGF-A and VEGF-C mRNA expression, respectively, in ARPE-19 A and HECV B cells cultured 24 h in standard conditions (CTR) or in the presence of AGEs (GS). GAPDH was used as the internal control. VEGF-A and VEGF-C secretion in ARPE-19 C, E: and HECV D, F: cells, respectively, after 24 h culture in CTR or in presence of AGEs (GS). The conditioned media were collected, and ELISA was performed. Concentrations of VEGFs were calculated from standards curves and normalized to total protein. The results are representative of at least three experiments (mean±SEM). *p<0.05; **p<0.01; ***p<0.001.