Figure 8. mRNA expression of UM cell cultures after bevacizumab treatment under hypoxic conditions. mRNA expression was measured with qPCR under hypoxic conditions and after 24 h of different doses bevacizumab treatment. Expression is demonstrated in normalized fold. Two cell cultures show a clear induction of VEGF-A expression after treatment with bevacizumab (culture two and three). GLUT-1 expression in UM cell cultures corresponds to the VEGF-A expression (bottom); correlation coefficient 0.974, p=0.0002. AVA1=equivalent human dose: 1.25 mg/ 5.46 ml; AVA10=10 times the equivalent human dose: 12.5 mg/ 5.46 ml; AVA20=20 times the equivalent human dose: 25 mg/ 5.46 ml; CO=control group: culture medium.