Figure 5. Involvement of HIF-1α in B16F10 cells after bevacizumab treatment under hypoxic conditions. Expression of HIF-1α was measured in B16F10 cells using an in-cell Western assay after cell exposure to hypoxic conditions for 24 h. Expression is demonstrated in normalized integrated intensity ((HIF-1α/cell count)*100). A significant induction of HIF-1α was observed when comparing the AVA10 treated cells with the control (p=0.03; top). qPCR analysis shows an induction of GLUT-1 expression in B16F10 cells treated with the high dose of bevacizumab (AVA20) in comparison to no treatment. Expression is demonstrated in normalized fold (bottom). AVA1=equivalent human dose: 1.25 mg/5.46 ml; AVA10=10 times the equivalent human dose: 12.5 mg/ 5.46 ml; AVA20=20 times the equivalent human dose: 25 mg/ 5.46 ml; CO=control group: culture medium.