Figure 3. Protein alignment for the novel missense mutations identified in FZD4 and LRP5. Nonconserved amino acid residues are boxed. The residues with mutations are highly conserved. FZD4 orthologs included Homo sapiens (NP_036325.2), Pan troglodytes (XP_001175326.1), Mus musculus (NP_032081.2), Rattus norvegicus (NP_072145.1), Bos taurus (NP_001193198.1), Equus caballus (XP_001489854.1), Canis familiaris (XP_848753.1), Gallus gallus (NP_989430.1), and Danio rerio (XP_002664771.1). The LRP5 orthologs are from Homo sapiens (NP_002326.2), Pan troglodytes (XP_508605.2), Mus musculus (NP_032539.1), Rattus norvegicus (NP_001099791.2), Bos taurus (XP_614220.3), Gallus gallus (NP_001012915.1), and Danio rerio (NP_001170929.1).