Primers used for PCR amplification and sequencing of FZD4 and LRP5 Abbreviations: E: Exon, F: Forward, R: Reverse, bp: Base pair. FZD4-E2 was amplified in three overlapping segments A, B, and C. Primer sequences for LRP5-E4 obtained from Gong et al. [31]. Sequencing primer for LRP5-E10-F is another primer TTCCTCCTCACCTGCTG. To access the data, click or select the words “Appendix 1.” This will initiate the download of a compressed (pdf) archive that contains the file.