Table 2 of Venkatesan, Mol Vis 2012; 18:2420-2437.


Table 2. The primer sequences used for validation of the key genes in the primary retinoblastoma tumors by qRT–PCR.

Gene name Sense/Antisense: Sequences
ETS oncogene family (ELK1) FP: 5′GAAGAATCACACCCTTGGAA3′
  RP: 5′GACAAAGGAATGGCTTCTCA 3′
G-2 and S-Phase expressed1(GTSE1) FP: 5′ACGTGAACATGGATGACCCTA3′
  RP: 3′GTTCGGGAACCGGATTATTTA5′
cyclin dependent kinase 6 (CDK6) FP: 5′CTGAATGCTCTTGCTCCTTT3′
  RP: 5′AAAGTTTTGGTGGTCCTTGA3′
E2Ftranscriptionfactor4,p107/p130binding(E2F4) FP: 5′GGGCAGAAGAAGTACCAGATTCA3′
  RP: 5′GCTCCATGCCTCCTTGTTCA3′
v – crk sarcoma virus CT10 oncogene homolog(avian; CRK) transcript variant FP: 5′CCGGGACAAGCCTGAAGAGC3′
  RP: 5′GGCCCACCCAGTGGCTGTGG3′
CDH1 FP: 5′TCGACACCCGATTCAAAGTGG3′
  RP: 5′TTCCAGAAACGGAGGCCTGAT3′
SNAIL homolog 1 (Drosophila; SNAI1) FP: 5′TATGCTGCCTTCCCAGGCTTG3′
  RP: 5′ATGTGCATCTTGAGGGCACCC3′
MMP 2 FP: 5′AGATCTTCTTCTTCAAGGACCGGTT3′
  RP: 5′GGCTGGTCAGTGGCTTGGGGTA3′
MMP 9 FP:5′GCGGAGATTGGGAACCAGCTGTA3′
  RP: 5′GACGCGCCTGTGTACACCCACA3′