Figure 2. Effect of small interfering RNA on the expression of HMGA2 in retinoblastoma (Y79, WERI Rb1) cells in vitro. A: western blot analysis demonstrates markedly reduced HMGA2 expression in Y79 cells treated with HMGA2-siRNA (lane 1), strong expression of HMGA2 in non-transfected Y79 cells (lane 2), strong expression in Y79 cells treated with scrambled siRNA (lane 3), strong expression of HMGA2 in non-transfected WERI Rb1 cells (lane 4), and markedly reduced HMGA2 expression in WERI Rb1 cells treated with HMGA2-siRNA (lane 5). B: western blot analysis demonstrates expression of histone (normalization control) in Y79 cells treated with HMGA2-siRNA (lane 1), in non-transfected Y79 cells (lane 2), Y79 cells treated with scrambled siRNA (lane 3), in non-transfected WERI Rb1 cells (lane 4), and in WERI Rb1 cells treated with HMGA2-siRNA (lane 5).