Figure 1. Effect of small interfering RNA on the expression of HMGA2 in RB Y79 cells in vitro. A: The mRNA levels of high mobility group A2 (HMGA2) in RB cells (Y79) treated with HMGA2 short interfering (si) RNA, namely, the siRNA.1 (Hs_HMGA2_6) sequence, led to a 4.65 log₂ ratio decrease, siRNA.2 (Hs_HMGA2_7) led to a decrease of 2.0, the siRNA.3 sequence led to an increase of 1.76 log₂ ratio when compared with HMGA2 mRNA levels in control (solid bar) and to a decrease of a 1.68 log₂ ratio, 4.175 log₂ ratio, an increase of a 2.65 log₂ ratio, respectively, to cells treated with scrambled (SCR) siRNA (dotted bars) at the end of 48 h. The error bars represent the standard deviation of triplicate values. B: The mRNA levels of high mobility group A2 (HMGA2) in Y79 cells treated with HMGA2 short interfering (si) RNA [siRNA.1 (Hs_HMGA2_6) sequence] led to a decrease of a 4.65 log₂ ratio, in WERI Rb1 led to a decrease of a 2.56 log₂ ratio when compared with HMGA2 mRNA levels in RB control cells (solid bar) and to a decrease of a 4.175 log₂ ratio, a 3.17 log₂ ratio, respectively, in RB (Y79, WERI Rb1) cells treated with scrambled (SCR) siRNA (dotted bars at the end of 48 h). The error bars represent the standard deviation of triplicate values.