Figure 5. Expression and distribution of heat shock protein 70 in RF/6A cells after laser irradiation. A-C: Unirradiated RF/6A cells. D-F: RF/6A cells were treated with 700 mW laser irradiation and followed by 18 h incubation. Cells were fluorescently labeled with heat shock protein 70 (Hsp70; center, red channel) and the nuclear marker diamidinophenylindole (left, blue channel). The right is an overlay of the two channels. A strong induction of Hsp70 was observed in cells treated with a laser power of 700 mW. The induced Hsp70 was present in both the cytoplasm and nucleus. Data are from one of three separate experiments with similar results.