Figure 4. Hyperglycemia induces [Ca2+]i-dependent
and CaMKll-dependent JNK phosphorylation and Fas protein
expression in RF/6A cells. RF/6A cells were incubated for 96 h
in serum-free medium containing 5.5 glucose (NG), 30 mM glucose
(HG), or HG plus 2-APB, KN93, or SP600125 as indicated, and
subjected to western blotting analysis for A: JNK, and
p-JNK and for B: Fas. β-actin served as the loading
control. JNK, p-JNK, and Fas levels were quantified by
densitometry analysis in each treatment group (right panels).
Bars represented mean±SD from at least three independent
experiments with seven cells per treatment group. * p<0.05
versus NG; # p<0.05 versus HG; #
p<0.05 versus NG.