Figure 3. Hyperglycemia promotes CaMKll activation in RF/6A cells. A: RF/6A cells were incubated for 96 h in a serum-free medium with 5.5 mM glucose (NG), 30 mM glucose (HG), or HG plus 2-APB, and subjected to western blotting analysis for CaMKll and p-CaMKll protein levels. β-actin served as loading control. B: CaMKll and p-CaMKll levels were quantified by densitometry analysis under each treatment condition. Bars represented mean±SD from at least three independent experiments with seven cells per treatment group. * p<0.05 versus NG; ^# p<0.05 versus HG; ^# p>0.05 versus NG.