Figure 5 of Adamus, Mol Vis 2012; 18:2323-2337.

Figure 5. Clearance kinetics of RTL220 conjugated to fluorescent IRDye 800CW observed in the RCS rat. Representative data for 2 groups of rats (pictured) administrated with a single or triple dose of fluorescent RTL220 subcutaneously and monitored over time for its distribution and clearance. A: The rat received a single dose of 50 μg of RTL and was imaged at 0, 10, 30, 60, and 120 min. B: Daily imaging of the rat illustrate a slow dispersion of the fluorescent RTL from the injection site. C: The rat received 3 doses of the fluorescent RTL220 every other day and was imaged at 0, 10, 30, 60, and 120 min after each injection; D: Clearance of RTL220 after 3-dose treatment is shown over time to determine complete clearance.