Figure 4. Representative vascular pathology is shown for retinal whole mounts stained with nicotinamide adenine dinucleotide phosphate (NADPH)-diaphorase to identify abnormal vessels associated with retinal pigment epithelium (RPE) cells (vascular complexes; arrows). A: Photomicrographs show the vascular complexes from middle part of the retina that were dramatically reduced in RTL220-treated rats compared to control rats. RTL101 was not effective and its effect was similar to vehicle- or plain interphotoreceptor retinoid binding protein (IRBP) peptide-treated RCS rats. B: Optic nerve head region view of RTL220 and control RTL101 treatment show the accumulation of pigmented cells around optic disc. C: Bar graph represents the overall reduction in vascular complexes in RTL220-treated retinas compared to vehicle-treated retinas (n=6, p=0.0004). D: Scheme of retinal flatmounts shows the area from which the abnormal vessels were counted (box).