Figure 2. Preservation of photoreceptors nuclei was present in RTL220-treated retinas at P90 when treatment started at P30. Treatment of Royal College of Surgeons (RCS) rats with 20 μg RTL220 every other day preserved the outer nuclear layer (ONL) thickness at P90. A: Confocal images of retinas stained with diamidino-phenyl-indole (DAPI) for nuclei at P90 from RTL220-, RTL101-, and vehicle-treated RCS rats were taken from peripheral and central close to the optic nerve head region. B, C: Graphs represent measurements of the total ONL density in histological sections of each treatment group that were recorded at 0.5, 1.0, 1.5, 2.0, 2.5, and 3 mm from the optic disc region in RTL220-treated rats (B) and control rats (C). The error bars represent SD (n=6, one-way ANOVA p=0.0022). Abbreviations: INL - inner nuclear layer, GCL - ganglion cell layer.