Figure 3. Cdh23 is expressed in GABAergic amacrine cells. In situ hybridization of cdh23 was combined with immunocytochemistry for various amacrine cell markers. In each row of images, the left panel shows retinal slices labeled with the cdh23 mRNA (mRNA) probe, the center panel shows fluorescent immunostaining, and the right panel shows an overlay of the in situ hybridization and the immunofluorescence images. A: cdh23-positive retinal cells colabel with the pan-amacrine cell antibody 5E11. B: cdh23-positive amacrine cells also colocalize with an antibody to the GABAergic cell marker glutamate decarboxylase 65/67 (GAD65/67). C, D: Neither serotonin-positive nor tyrosine hydroxylase (TH)-positive amacrine cells colabel with cdh23 mRNA. Scale bar, 20 µm.