Appendix 1 of Glover, Mol Vis 2012; 18:2309-2322.


Appendix 1.Expression of cdh23 mRNA in wild-type and mutant retinas.

To access the data, click or select the words “Appendix 1.” This will initiate the download of a compressed (jpg) archive that contains the file. In situ hybridization using a probe directed against either the 5′- or 3′ region of cdh23 showed that cdh23 mRNA (mRNA) is expressed in a subset of amacrine cells of both cdh23tc317e siblings (left panels) and homozygous mutants (right panels). The 5′ RNA probe used in (A, B) was complementary to nucleotides 448–1,566 from cdh23 cDNA. C, D: The same amacrine cell staining of cdh23tc317e siblings (C) and mutants (D) was observed using a 3′ RNA probe complementary to nucleotides 9730–10,515.