Figure 3. siRNA mediated EpCAM downregulation and its effect on miR 17–92 expression. A: The western blot shows the down-regulation of EpCAM in Y79 cells treated with EpCAM specific siRNA. The lower panel shows the Beta actin expression as loading controls. B: QPCR shows the change in the expression of the miR 17–92 cluster in Y79 cells treated with EpCAM siRNA. There is a significant down-regulation of the miR 17–92 cluster by 4 to eightfold (*p<0.01).