Table 1 of
Haghighi, Mol Vis 2012; 18:211-218. Table 1 of
Haghighi, Mol Vis 2012; 18:211-218.
Table 1. PCR primers and conditions.
Set |
Forward sequence (5’→3’) |
Melting temperature (°C) |
Reverse sequence (5’→3’) |
Melting temperature (°C) |
PCR product size (bp) |
|---|---|---|---|---|---|
| FOXL2 primers | |||||
| 1 | ctaggggaaggggaaggag | 60.0 | gttgtggcggatgctatttt | 60.0 | 500 |
| 2 | cgaagttcccgttctacgag | 59.9 | gcatagggcatgggtgag | 60.0 | 391 |
| 3 | gacggctacggctacctg | 59.4 | ccaggccattgtacgagttc | 60.5 | 283 |
| 4 | ccggcgtagtgaactcgta | 59.9 | aaagcgaaaaagcacagagg | 59.6 | 486 |
| 5’ UTR primer | |||||
| 1 | ctccttgactgtgcgac | 53.1 | aaagtgacttggagatgaact | 53.0 | 594 |
| 3’ UTR primers | |||||
| 1 | gagcgacagaaataaagaagtcc | 58.6 | ttcaaacctcctgcttctcc | 59.4 | 271 |
| 2 | cgggtttcacatttctcctt | 59.0 | ggaagtattgtggccttgga | 59.9 | 366 |
| 3 | gattttcatatttggatttagcaaac | 58.6 | gccggacaggactgatgg | 62.7 | 400 |
| 4 | cggagcaaacacacgtattg | 60.2 | agggtccctctgtgcttttt | 60.1 | 390 |
| 5 | gagcgacaggagcttaggaa | 59.7 | gccatgatgcattgctctta | 59.8 | 247 |