Table 1 of
Lu, Mol Vis 2012; 18:2255-2264. Table 1 of
Lu, Mol Vis 2012; 18:2255-2264.
Table 1. PCR Primers.
| Gene | Primer sequence (5′→3′) | Product size (bp) | Annealing temperature | GenBank accession ID |
|---|---|---|---|---|
| Keratocan | Forward: TCTGTCCTCCCAGTTTCC | 205 | 50 °C | NM_008438 |
| Reverse: CCCTTCTCAATCCCGTAG | ||||
| ALDH | Forward: GGAGCACGGCTGTAGGAA | 325 | 52 °C | NM_007436 |
| Reverse: TTTATGACCCGCTGGAAG | ||||
| TGF-β2 | Forward: GGGGTTAAGGAGGTGG | 229 | 57 °C | NM_009367 |
| Reverse: GGAGACTCGGTGGGATA | ||||
| PTGS2 | Forward: CTCTGCGATGCTCTTC | 268 | 53 °C | NM_011198 |
| Reverse: AATGTTCCAGACTCCCT | ||||
| M-CSF | Forward: ATGATGCCAAGAGGAAGACA | 159 | 54 °C | NM_007778 |
| Reverse: CATAGGGATGGATGGGACA | ||||
| IL-10 | Forward: ACCAAAGCCACAAAGCAG | 283 | 54 °C | NM_010548 |
| Reverse: GGCAACCCAAGTAACCCT | ||||
| β-actin | Forward: GGAAATCGTGCGTGACATTA | 379 | 54 °C | NM_007393 |
| Reverse: GGAGCAATGATCTTGATCTTC |