Figure 3. Cytopathic effect of Acanthamoeba on corneal fibroblasts in various conditions. A: Acanthamoebae (0 to 10×10³) were added to corneal fibroblasts in each well and incubated at 25 °C for 2 days. Acanthamoebae (1×10⁴) significantly decreased the viability of corneal fibroblasts compared with no Acanthamoebae (n=4). B: A significant decrease of corneal fibroblast viability was detected from day 2 (n=4). C: Cytopathic effect on corneal fibroblasts for 6 Acanthamoebae isolates from our AK patients. A significant decrease of optical density (indicating a cytopathic effect on corneal fibroblasts) was detected with all tested Acanthamoebae compared to control cultures with no Acanthamoebae (n=4). Similar findings were obtained with repeated two experiments. Representative data are shown. *p<0.05.