Figure 2. Electron micrograph of the renal biopsy and indirect immunofluorescence staining of the skin biopsy. Electron micrograph of the renal biopsy of the patient II-2 (A) showed irregular thinned and thickened areas of the GBM with splitting of the lamina densa (magnification, 8,000×). Indirect immunofluorescence staining of the skin biopsy using murine monoclonal antibodies against the NC domain of collagen α5 (IV) and α1 (IV; Wieslab, Lund, Sweden) showed that III-1 was positive for the α1 (IV) chain in a linear continuous pattern (B; positive control) and negative for the α5 (IV) chain (C), respectively (magnification, 200×).