Figure 4 of Acharya, Mol Vis 2012; 18:2182-2189.


Figure 4. Confirmation of interaction between PITX2 and EFEMP2. Left Panel: The Xpress-PITX2A protein was immunoprecipitated with anti-Xpress antibodies and V5-EFEMP2ΔN36 was detected with anti-V5 antibody. V5-EFEMP2ΔN36 was not immunoprecipitated in the absence of the PITX2 (XpressEmpty control). Right Panel: In a reciprocal experiment, V5-EFEMP2ΔN36 was immunoprecipitated with anti-V5 antibodies and Xpress-PITX2A was detected with anti-Xpress antibody. Similarly, Xpress-PITX2 was not immunoprecipitated in the absence of the EFEMP2(V5Empty control). Twenty percent of the amount of cell lysate used for immunoprecipitation was loaded on the SDS–PAGE for the “input” lanes.