Figure 4. Confirmation of interaction
between PITX2 and EFEMP2. Left Panel: The Xpress-PITX2A protein
was immunoprecipitated with anti-Xpress antibodies and V5-EFEMP2ΔN36
was detected with anti-V5 antibody. V5-EFEMP2ΔN36 was
not immunoprecipitated in the absence of the PITX2 (XpressEmpty
control). Right Panel: In a reciprocal experiment, V5-EFEMP2ΔN36
was immunoprecipitated with anti-V5 antibodies and Xpress-PITX2A
was detected with anti-Xpress antibody. Similarly, Xpress-PITX2
was not immunoprecipitated in the absence of the EFEMP2(V5Empty
control). Twenty percent of the amount of cell lysate used for
immunoprecipitation was loaded on the SDS–PAGE for the “input”
lanes.