Figure 3. Detection of EFEMP2 and PITX2 expression in ocular cell lines by RT–PCR. Intron-flanking primers were used to amplify mRNA transcripts of EFEMP2 (161 bp) and PITX2 (133 bp) from various transformed cell lines, using the housekeeping gene HPRT1 (130 bp) as a control. Reverse-transcribed (RT) samples either contained (+) or lacked (-) enzyme, and control templates of genomic DNA and water were included, to verify that amplified products were a result of specific primer binding.