Figure 3. Imaging of mitosis of the LECs. Numerous mitotic figures were present all over the anterior surface after 15 h of incubation with 20 ng/ml IGF-I (A). The chromosomes and the profiles of the cell bodies were distinctly visible, and dividing cells in various stages could easily be observed (B–J). A vague, dark ring (arc) was located in the center of the lens surface (A). The curved surface of the lens permitted visualization of a small area of cells in the focal plane. Scale bars A=50 μm; B–J=10 μm.