Figure 3. Example of TO-PRO-3 images in BALB/cByJ control and optic nerve crush (ONC) retinas. Panel A and panel B demonstrate the TO-PRO-3 staining in the BALB/cByJ control and ONC retina, respectively. Only the RGC layer cells that were not obviously endothelial cells were counted. There were four individual retinas with approximately 12 fields each, totaling 48 counted fields per control and ONC group. The scale bar is 50 µm.