Figure 2. Optical coherence tomography Doppler imaging before and after optic nerve crush (ONC). OCT was used to measure blood flow using Doppler imaging. We were able to confirm that there is continued perfusion of the retina before and after ONC. Panel A shows the OCT Doppler image of the retina immediately before ONC, and panel B illustrates the blood flow immediately after ONC. The arrows point to some of the vessels present on the surface of the retina. The colors reflect the direction of blood flow; red is flow toward the transducer and blue is flow away from the transducer. These data demonstrate that in the mouse this procedure does not interrupt blood flow to the retina. The abbreviations in the figures are defined as follows: retinal pigment epithelium (RPE), outer nuclear layer (ONL), inner nuclear layer (INL), and ganglion cells (GC).