Figure 7. Western blot analysis of p38 MAPK in tBHP treated iHTM cells. The p38MAPK and phosphorylated p38MAPK were detected by western blotting with p38 MAPK (p38) and phospho-p38 MAPK (p-p38) antibodies. The same samples were probed with GAPDH as a loading control (A). The p38MAPK levels were of little difference among all cells. The p-p38 levels increased after exposure to tBHP without pretreatment with SB203580, especially in the cells treated for 2 h. Nevertheless, in the SB203580 (+) groups, the level of p-p38 substantially decreased. There were nearly no p-p38 could be detected in the cells pretreated with SB203580 (B).