Figure 5. Apoptosis of iHTM cells detected by flowcytometry after tBHP exposure with or without pretreatment with SB203580. iHTM cells not treated with tBHP or SB203580 were set as control (A). Cells treated with tBHP for 1 h (B) and 2 h (C) without SB203580 pretreatment. Cells were pretreated with SB203580 for 30 min and then treated with tBHP for 1 h (D) and 2 h (E). The rate of apoptosis in SB203580(-) cells and SB203580(+) cells after exposure to tBHP for 1 h were 28.23±3.23% (B) and 12.75±1.91% (D), those in SB203580(-) cells and SB203580(+) cells after exposure to tBHP for 2 h were 39.20±5.91% (C) and 20.40±3.44% (E). The rate of cell death and apoptosis in SB203580(-) cells were higher than SB203580(+) cells (p<0.05). The data were presented as mean±SD, n=4, p<0.05.