Figure 1. Morphology of iHTM cells
after tBHP exposure for 1 h and 2 h with or without
pretreatment with SB203580. iHTM cells were not treated with tBHP
or SB203580 (A). Cells were not pretreated with SB203580
and just treated with tBHP for 1 h (B) and 2 h (C).
Cells were pretreated with SB203580 for 30 min and then treated
with tBHP for 1 h (D) and 2 h (E). More
cells from SB203580(-) group became rounded, shrank and
dettached after treatment with 200 μM tBHP for 1 h as
compared to those from SB203580(+) group. Gross morphological
changes of cells were more apparent after tBHP treatment
for 2 h. Phase-contrast light microscope 100×.