Figure 5. Effects of p38
mitogen-activated protein kinases (MAPK) inhibitor and nuclear
factor kappa-light-chain-enhancer of activated B cells (NF-κB)
siRNA on nucleosome release. The apoptosis of retinal tissues
was evaluated by nucleosome release ELISA. Eyes injected with
SB203580 or NF-κB p65 siRNA had higher nucleosome
release than the ischemia/reperfusion (I/R) control groups.
Control: normal retinas, the other five groups were individually
as I/R, I/R+vehicle (0.1% DMSO), I/R+control of siRNA in vehicle
(Lipofectamine 2000 [Invitrogen] and DMEM/F12: no serum and no
antibiotics), I/R+SB203580 and I/R+NF-κB siRNA.
*Statistically significant compared to the I/R control:
p<0.05. ** Statistically significant compared to the normal
control: p<0.05. n=6 in each group. Cell number was given as
mean±standard deviation (SD).