Table 1 of
Batista, Mol Vis 2012; 18:194-202. Table 1 of
Batista, Mol Vis 2012; 18:194-202.
Table 1. RT–PCR parameters for SNARE elements.
Gene |
Accession number |
Primer sequence |
Number of cycles |
BP size |
Annealing temperature |
|---|---|---|---|---|---|
| Actb | NM_031144 | Sense: 5′-agagggaaatcgtgcgtgaca-3′ | 33 | 202 | 59 °C |
| Antisense: 5′-cgatagtgatgacctgaccgtca-3′ | |||||
| Rab3D | NM_080580 | sense: 5′-actgatggtgacaatgatgc-3′ | 37 | 340 | 59 °C |
| antisense: 5′-acggaagtgaagaaagcaac-3′ | |||||
| Rab27b | NM_053459 | sense: 5′-cggagctcgagaagactaga-3′ | 37 | 225 | 60 °C |
| antisense: 5′-ggccaggagtttaatcaggt-3′ | |||||
| Vamp2 | 012663 | Sense: 5′-gcatctctcctaccctttca-3′ | 34 | 141 | 58 °C |
| Antisense: 5′-tttaggggtctgagggtaca-3′ |