Figure 5. Effect of L-MSC on CD3/CD28 stimulated PBMC. A: CD3/CD28 stimulated PBMC were cultured alone or in indirect contact (in a transwell system) with decreasing concentrations of L-MSC (L-MSC: PBMC ratio), non-stimulated cells are also shown on left bar. B: CD3/CD28 stimulated PBMC were performed alone or in the presence of supernatants from L-MSC cultures. Supernatants were collected from day 1 to day 5 L-MSC cultures and added (30% v/v) to the cell cultures. Data are expressed as mean±SEM from three independent experiments (* p<0.05).