Figure 4. Transdifferentiated L-MSC expressed tissue specific antigens. Micrographs of immunofluorescence identifying nestin and collagen-II antigens. L-MSC were cultured in differentiation media (see materials and methods) and immunofluorescence was performed to identify nestin protein (neurogenesis) in A, and collagen-II (chondrogenesis) in B. Cells were DAPI counterstained (40×).