Figure 2. Clonogenic properties and proliferative potential of L-MSC. The cells were cultured for 10 days and stained with 0.5% crystal violet in methanol. A colony-forming unit was determined when measured >2 mm (arrow). Colonies that measured <2 mm or faintly stained were excluded (A). B: Representative growth curve of L-MSC as a function of time after the culture. Data are expressed as mean±SEM from three independent experiments.