Figure 3. Semi-quantitative reverse transcription -PCR analysis of plasma membrane calcium ATPase 1 (PMCA1) mRNA in rat lenses visualized on an ethidium bromide-stained agarose gel alongside mRNA of β-actin (ACTB). A: M- 100 bp DNA ladder; GI- Group I (Normal); GII- Group II (Selenite-challenged, cataract-untreated); GIII- Group III (Selenite-challenged, acetyl-L-carnitine -treated). B: The results depicted are normalized to levels of ACTB. Data are mean value (experiments run in triplicate) of ratios of intensity for gene of interest divided by that for ACTB. ^aGroup I versus Group II & III values (p<0.05); ^bGroup II versus Group III values (p<0.05).