Figure 2. Semi-quantitative reverse
transcription -PCR analysis of connexin 50 mRNA in rat lenses
visualized on an ethidium bromide-stained agarose gel alongside
mRNA of β-actin (ACTB). A: M- 100 bp DNA ladder;
GI- Group I (Normal); GII- Group II (Selenite-challenged,
cataract-untreated); GIII- Group III (Selenite-challenged,
acetyl-L-carnitine-treated). B: The results depicted are
normalized to levels of ACTB. Data are mean value
(experiments run in triplicate) of ratios of intensity for gene
of interest divided by that for ACTB. aGroup
I versus Group II & III values (p<0.05); bGroup
II versus Group III values (p<0.05).