Table 1 of
Mendoza-Reinoso, Mol Vis 2012; 18:2067-2075.
Table 1 of
Mendoza-Reinoso, Mol Vis 2012; 18:2067-2075.
Table 1. Primer pairs 1F-2R and 3F-ST3R were used to amplify MYOC exon 3 in two segments to be screened by CSGE, and pair 1F-4R to amplify the entire exon for sequencing.
Primer |
Sequence |
Annealing Temp. (°C) |
Product length (bp) |
|---|---|---|---|
| 1F | 5’-CTGGCTCTGCCAAGCTTCCGCATGA-3’ | 60 | 480 |
| 2R | 5’-GAGGCCTGCTTCATCCACAGCCAAG-3’ | ||
| 3F | 5’-GAGCTGAATACCGAGACAGTGAAGGC-3’ | 56 | 586 |
| ST3R | 5’-GAAAGCAGTCAAAGCTGCC-3’ | ||
| 1F | 5’-CTGGCTCTGCCAAGCTTCCGCATGA-3’ | 60 | 967 |
| 4R | 5’-GGCTGGCTCTCCCTTCAGCCTGCT-3’ |