Table 2 of
Kapoor, Mol Vis 2012; 18:2022-2032. Table 2 of
Kapoor, Mol Vis 2012; 18:2022-2032.
Table 2. Details of PCR primers used in mutation analysis of the EDN3 gene.
| Exon | Primer | Primer sequence (5′ to 3′) | Tm (°C) | Amplicon size (bp) |
|---|---|---|---|---|
| 1* | EN1bF | F:gaaaagcccgagccacagccggc | 64 | 379 |
| EN1bR | R:ccgcgacgcacatcttctccgcg | |||
| 2 | EN2F | F:cagacattttgcttgctccacc | 62 | 528 |
| EN2R | R:caggctctgggctaactgagc | |||
| 3 | EN3F | F:ggcggtggttctcgctccacac | 56 | 372 |
| EN3R | R:caggatgtgactgaactatccta | |||
| 4 | EN4F | F:tggggaacgcactaatgtgctca | 62 | 336 |
| EN4R | R:agaaacggtccaccaaaggcacc | |||
| 5 | EN5F | F:ttccagtctggtggtaggctcg | 56 | 458 |
| EN5R | R:gtattgttaagtggggactctttg |