Figure 3. Wnt3a promotes the migration of HLE B-3 cells. A: Wound healing assay showed increased migration ability of HLE B-3 cells after 48 h of transfection with Wnt3a-pcDNA3 plasmid. The images were taken at 0 and 24 h after scratch was applied (left; original magnification 100×). The migration distance of the Wnt3a-overexpressing cells at different time points was significantly increased compared with that of control cells (right; p<0.05). B: Transwell migration assay. Cells on transwell inserts were stained (left; original magnification 200×). The number of migratory Wnt3a-overexpressing HLE B-3 cells was significantly higher than that of control cells (right; p<0.05).