Figure 1. Wnt3a activates the Wnt/β-catenin signaling pathway in HLE B-3 cells. A: western blot analysis showed that overexpression of Wnt3a results in the upregulated expression of Wnt/β-catenin signaling component proteins, including β-catenin, c-Myc, and cyclin D1. β-Actin was used as internal control. B: Immunocytofluorescence also demonstrated increased expression of β-catenin protein (red) in Wnt3a-overexpressing cells compared with control cells. Nuclei were stained with DAPI (blue). Merged images are shown in the right panel.