Figure 5. Effect of caspase-14 expression on the levels of cleaved PARP-1 in retinal ECs. Western blot analysis of caspase-14 in BRECs infected with adenovirus expressing caspase-14 and GFP (Ad-casp14; A) or GFP only (Ad-GFP; B) demonstrated overexpression of caspase-14 in Ad-casp14 compared to the Ad-GFP. Please note that overexpression of caspase-14 in BRECs was associated with a significant increase in the cleaved PARP-1 compared to the control cells infected with Ad-GFP (C; *p<0.05).