Figure 3. Caspase-14 in the mouse retina. Immunofluorescence reaction (A) using specific antibody against caspase-14 (green), vascular marker (isolectin B4-red), and nuclear stain (DAPI) showed marked increase in the expression of caspase-14 in different layers in particular in relation to retinal vasculatures (red). Western blot analysis of caspase-14 in the mouse retina (B) demonstrated significant upregulation in diabetic mice and the presence of cleaved caspase-14 in one of the diabetic mice compared to the control (C). (* p<0.05; D versus C; n=6).