Figure 1. Expression of caspase-14 in
the retinal cells. Western blot analysis of retinal cell lysates
showed that caspase-14 is present (A) and is processed
under reduced conditions in the presence of β-mercaptoethanol (B)
in different retinal cells including pericytes (PCs),
endothelial cells (RECs), pigment epithelium (RPE) cells, and
astrocytes (ACs). Lung ECs and trabecular meshwork (TM) cells
also expressed caspase-14. qPCR analysis of caspase-14 mRNA (C)
demonstrated the presence of caspase-14 mRNA in the same cells
and in choroidal ECs. Please note the higher expression of
caspase-14 in PC and RPE cells compared to other retinal cells.
The cell culture experiments were repeated using two different
isolations with similar results.