Figure 4. Immunofluorescent staining with TNF-α specific antibody (green) in corneal epithelia (CE: arrows) and conjunctival epithelia (CJE: arrowheads) during the observation period. Nuclear counterstain in blue. Increased staining intensity for TNF-α in CE and CJE was detected in BTX-B injected mice (1, 2, and 4 weeks post-injection). Very weak staining in CE and CJE was observed in normal and saline-injected mice during the observation period (F-J and P-T with nuclear counterstain in blue). Images of isotype and negative controls were omitted (non-staining). Scale bar: 50 µm.