Figure 3. Immunofluorescent staining with IL-1β antibody (red) in corneal epithelia (CE: arrows) and conjunctival epithelia (CJE: arrowheads) during the observation period (F-J and P-T with nuclear counterstain in blue). Increased staining intensity for IL-1β in CE and CJE was shown in BTX-B injected mice (1, 2, and 4 weeks post-injection), with most intense labeling at 2 weeks post-injection. Very weak staining in CE and CJE was detected in normal and saline-injected mice. Negative and isotype controls, which did not stain, were omitted. Scale bar: 50 µm.