Molecular Vision: Piñeiro-Gallego, Mol Vis 2012; 18:1794-1802. Table 1

Table 1. Oligonucleotides for genomic amplification and sequencing of exon 8, 10, and 16 of ALMS1 and exon 6 of RPGRIP1L.

Gene	Exon	Forward Primer (5′-3′)	Reverse Primer (5′-3′)	Product size (bp)	Tm (°C)
ALMS1	8.1	GCTTTTTAAAGGCTCAAAGCTG	GTATTCCCGTCTTCTGCTCCACT	646	52
	8.2	CAACTGGCATGTCAACTC	CTTCGGGTAGATGGCTGTC	666	56
	8.3	GTACCCACAGGACTTAGCA	ACTCCTGTTGATAGAAAATACTGG	731	58
	8.4	CTGACCAGAAGACTGTCCCAACAC	CAAGGCCTGCTGGTGGAAAAT	515	64
	8.5	CACACCAGCAGTACCGTCTAC	TCAGAGCCTCTTCAGTTGGATGATTA	527	66
	8.6	GAAAGTTTCACCTGTTCTTG	TGGTCCAGGAGCAGAAGAA	1021	59
	8.7	TGGCGCACCAACTATAACCTCTC	GCTGGTAGAAAATGACAGGCTTCC	491	64
	8.8	CTAAATAAAGAGGTTGTGAAAG	ATGTGAATAGAAAGAGGAAGTTA	375	53
	8.9	CAGGCCCTGCCAGACAGTGAG	CTGGCAACTCCTGCTGATGAAA	565	63
	8.1	GGTTCCTGGGCCTGCTGAC	TTGGGCTTTACTGTTTGAGAATAG	534	61
	8.11	TCACAAATAGAGAAGCCCAAGAT	ATGTGAAAAGGAACTAGGAAGAGC	375	54
	8.12	ATGTAACTGAAGATGTGCTGAAG	TTCTGCCTCCATCAAAAGTGTC	473	61
	8.13	AAGGATTTGCCAGATAGACAT	TCCTCTGTGAATGGCTGTCTGT	515	53
	8.14	ACTCTTAAGGAAATTCGGACAC	TTACAGATTTGGCTGCTTGATA	391	54
	8.15	CCTCTTCCACGGGTGTATCTAA	TGGCTAAGCTTCCTCAAAACA	710	56
	10.1	TGGTCTAATCTTAGCGTGGGT	ATGCATAGTAATTCACAAG	774	55
	10.2	ACACCATTTTCCCCTTCCT	CCGTGTGATTTCTCTGAGTGG	706	59
	10.3	TGTACTGGAGCATCTGTGGG	CAGTCAGCCCCAAATCACTG	860	63
	16.1	GCGAGGCTACTAAGCAACAAGGC	ACAGCCTGAGTTGGGTGAC	650	60
	16.2	CAGAAGGTCACCCCAGAG	CCATCTGGCAATGTGACTGC	835	63
RPGRIP1L	6	TTCACTGTGTGCAGAGGCACTT	TGCTCGACCTACTAACTGCTGTCA	447	62