Molecular Vision: Brennan, Mol Vis 2012; 18:1773-1786. Table 1

Table 1. Oligonucleotide primers used in semi-quantitative RT–PCR.

Gene	Forward primer	Reverse primer	NCBI#	Cycles	ng RNA	Annealing temp
beclin 1	CGGGAAGTCGCTGAAGACAG	CCATCCTGGCGAGGAGTTTC	NM_003766.3	30	100	55
atg14	GAGCGGCGATTTCGTCTACT	CTGAAGACACATCTGCGGGG	NM_014924.4	35	50	55
mtor	TTCTGGTGCGACACCGAATC	CATCGGGTTGTAGGCCTGTG	NM_004958.3	30	100	55
rb1cc1/fip200	GGAGCTTGTGCACCTGAACT	GAAGCACCCTCACCTGGTTTG	NM_014781.4	35	50	55
ralb	GCTCGTCGTGGGAAACAAGT	TGACAAAGCAGCCCTTCCAC	NM_002881.2	35	50	55
atg4a	GAGTAAGGGCACCTCTGCCTA	GTTCATTCGCTGTGGGGACT	NM_052936.3	35	50	55
atg12	GAGGTCTGTAGTCGCGGAGA	TGGATGGTTCGTGTTCGCTC	NM_004707.3	30	100	55
map1lc3b /atg8	AAGTGGCTATCGCCAGAGTCG	CTGAGATTGGTGTGGAGACGC	NM_022818.4	25	100	55
rab7	GACACAGCAGGACAGGAACG	TTGTACAGCTCCACCTCCGT	NM_004637.5	25	100	55
fyco1	GAAGCTGAAGGCCACCCAAG	GGGCATCTGACTTCTGCCAG	NM_024513.3	35	50	55
bnip3l/nix	ACTCGGCTTGTTGTGTTGCT	TCCCTGCTGGTGTGCATTTC	NM_004331.2	25	100	58
pink1	TCTGCAGTCCTCTGCTCACA	GCTCATCCGTCACTTTCGCT	NM_032409.2	35	50	55
p62	CTCACCGTGAAGGCCTACCT	TAGCGGGTTCCTACCACAGG	NM_003900.4	35	50	55
GAPDH	CCACCCATGGCAAATTCCATGGCA	TCTAGACGGCAGGTCAGGTCCACC	NM_003900.4	35	50	60