Figure 3. LC3B levels in serum starved human lens epithelial cells. A: LC3B levels in lens epithelial HLEB3 cells exposed to serum starvation and chloroquine (50 µM) detected by immunoflourescent confocal imaging (green). The nucleus is shown by DAPI staining (blue). B: Mean number of LC3B puncta are shown for each treatment (n=50 cells; error bars represent standard deviations). The data are statistically significant at p<0.001 by Tukey analysis.