Figure 2. Spatial localization of LC3B and FYCO1 in whole mouse lenses. Immunostaining of LC3B and FYCO1 in postnatal day 1 mouse lens with LC3B-specific antibody and FYCO1-specific antibody. Secondary antibody alone is shown as control. Lens epithelium (LE), lens cortical fibers (CF) and nuclear fibers (NF) are indicated. Brown staining show positive antibody cross reactivity and blue hematoxylin staining is nuclear staining.