Table 2 of
Chen, Mol Vis 2012; 18:1763-1772. Table 2 of
Chen, Mol Vis 2012; 18:1763-1772.
Table 2. Primer sequences and thermal cycler conditions for NTF4 sequence analysis.
| Primer sequence | |||||
|---|---|---|---|---|---|
| Amplifying target | Forward primer (5′→3′) | Reverse primer (5′→3′) | MgCl2 (mM) | Ta (°C) | Size (bp) |
| NTF4–1 | ACTTGAAGAGGAACTCTGGGAAG | TCAAAACTGCCACTAAGGAGGTA | 1.5 | 58 | 412 |
| NTF4–2A | CTTCTTTCCCCACTGAAGTTTTT | CACCTTCCTCAGCGTTATCAG | 1.5 | 58 | 524 |
| NTF4–2B | CCCCGAGTAGTCCTGTCTAGG | CTCTCAGCATCCAGCTCTGTTAT | 1.5 | 58 | 544 |